FIG 3.

BCDA inhibits incorporation of d-Glu into nascent peptidoglycan precursors. (A) Fold change in intracellular levels of PG precursors in H37Rv, relative to untreated control cultures, following 24 h of challenge with various compounds at the specified concentrations. No MIC could be determined for vancomycin (VAN) using the filter-growth method, and therefore a concentration of 50 μg ml⁻¹ was chosen based on published results (16). Translucent gray-shaded bars represent equivalent values measured in B. subtilis. Results are the averages ± SEM from at least duplicate independent experiments. (B) Fold change in MIC for H37Rv (left window) following supplementation of growth media with 2 mM l-Ala, d-Ala, l-Glu, or d-Glu. Data for l-Ala and d-Ala are taken from Fig. 2 and are shown here again for comparison. The right window shows the fold change in the BCDA MIC for four alternative bacterial species following supplementation of media with 2 mM d-Glu. See also Fig. S3 in the supplemental material.