FIG 4.
Effects of ML:8 and Citrox on in vivo biofilms formed over 5 days. Optimized doses of 106 CFU/ml (A and B) and 104 CFU/ml (C and D) MRSA USA300 lux in 40 μl were injected into catheters, and infection was allowed to develop for 1 (C and D) or 5 (A and B) days. This was followed by treatment for 4 days with saline (control), ML:8 (1%), or Citrox (1%). Animals were euthanized at day 9. Catheters were explanted, bioluminescence activity was acquired using an IVIS 100 camera (A and C), and log10 CFU counts were determined (B and D). Representative catheters are shown. Three animals were used per group, and data represent mean log10 CFU per milliliter and SD. Statistically significant results are indicated (*, P ≤ 0.05; two-tailed Student t tests versus untreated animals).