FIG 3.

Impact of subinhibitory concentrations of clindamycin on the expression of biofilm-associated genes. Relative transcript levels of genes important for eDNA and autolysis (A) or stress response and adhesion (B) derived from LAC wt biofilm mass with and without clindamycin after 18 h as determined by qRT-PCR. Fold change ratios were calculated by normalizing cDNA levels of the gene of interest against the housekeeping gene gyrB. Data are presented as fold change compared to levels in biofilms grown without clindamycin (set to 1), and error bars indicate interquartile range of at least three independent experiments. The dotted line indicates a 2-fold upregulation.