FIG 1.

Tyrphostin AG1478 inhibits EMCV replication. (A) Chemical structures of AG1478 and the PI4KA inhibitor AL-9. Outlined is the common 4-anilino-quinazoline core. (B) Sensitivities of EMCV and CVB3 to AG1478 treatment. HeLa cells were infected with for 30 min with EMCV or CVB3 at a multiplicity of infection (MOI) of 1, after which virus-containing medium was replaced with fresh (compound-containing) medium. Cells were freeze-thawed to release intracellular virus particles, and the total virus titers at 0.5 (input) and 8 h postinfection (hpi) were determined by endpoint dilution. The cytotoxicity of the treatment was measured separately in a cell viability assay. Shown are mean values ± standard errors of the means (SEM). Means were statistically compared using the unpaired t test. TCID₅₀, 50% tissue culture infective dose; **, P < 0.01; ***, P < 0.001.