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Tachyplesin I induces increased extracellular proteolytic activity in bacteria. Concentrated culture filtrates of cultures grown with and without 20 μg/ml tachyplesin I for different times were pipetted into holes in skim milk agar plates and incubated. The assays were performed in triplicate. Clearing zone data represent clearing zone diameters minus the 0.2-cm hole diameter. All data represent means and standard deviations.
