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. 2016 Sep 23;60(10):6067–6075. doi: 10.1128/AAC.00640-16

FIG 4.

FIG 4

Zymographic analysis of extracellular proteases in P. aeruginosa induction strains. Culture filtrates (20 μl) were analyzed in SDS-PAGE gels copolymerized with skim milk. After electrophoresis, gels were washed, incubated, and counterstained. (A) Culture filtrates of P. aeruginosa-80 resistant and control strains after different times. Lanes: 1, control strain (12 h); 2, resistant strain (12 h); 3, control strain (24 h); 4, resistant strain (24 h). Images for 12 h and 24 h were taken from different areas of the same gel. (B) Culture filtrates of P. aeruginosa resistant strains obtained by UV mutagenesis. Lanes: 1 to 4, P. aeruginosa UV-2, -3, -4, and -6 resistant strains (12 h); 5 to 8, P. aeruginosa UV-2, -3, -4, and -6 resistant strains (24 h).