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. 2016 Sep 23;60(10):6067–6075. doi: 10.1128/AAC.00640-16

FIG 5.

FIG 5

Resistant strains of P. aeruginosa regulate degradation of tachyplesin I (TacI). Equal amounts of peptide were incubated with culture filtrates of the control strain and the P. aeruginosa-80 and UV-induced resistant strains for 5 h and then analyzed by SDS-PAGE. (A) Culture filtrates of P. aeruginosa-80 resistant and control strains regulate degradation of TacI for different times. Lanes: 1 and 2, control strain and control strain plus TacI (12 h); 3 and 4, resistant strain and resistant strain plus TacI (12 h); 5 and 6, control strain and control strain plus TacI (24 h); 7 and 8, resistant strain and resistant strain plus TacI (24 h). (B) Culture filtrates of UV-induced P. aeruginosa resistant strains regulate degradation of TacI for different times. Lanes: 1 to 4, UV-2 plus TacI, UV-3 plus TacI, UV-4 plus TacI, and UV-6 plus TacI (12 h); 5 to 8, UV-2 plus TacI, UV-3 plus TacI, UV-4 plus TacI, and UV-6 plus TacI (24 h).