TABLE 3.
Antimicrobial agent | Drug content of assay paper (/piece) |
E. coli-96 resistant strain |
E. coli-96 control strain |
F41-80 resistant strain |
F41-80 control strain |
A. hydrophila-69 resistant strain |
A. hydrophila-69 control strain |
P. aeruginosa-80 resistant strain |
P. aeruginosa-80 control strain |
P. aeruginosa original strain |
P. aeruginosa UV-1 resistant strain |
P. aeruginosa UV-3 resistant strain |
P. aeruginosa UV-7 resistant strain |
UV-induced E. coli resistant strain |
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Inhibitory zone diam (mm) | Judgment result | Inhibitory zone diam (mm) | Judgment result | Inhibitory zone diam (mm) | Judgment result | Inhibitory zone diam (mm) | Judgment result | Inhibitory zone diam (mm) | Judgment result | Inhibitory zone diam (mm) | Judgment result | Inhibitory zone diam (mm) | Judgment result | Inhibitory zone diam (mm) | Judgment result | Inhibitory zone diam (mm) | Judgment result | Inhibitory zone diam (mm) | Judgment result | Inhibitory zone diam (mm) | Judgment result | Inhibitory zone diam (mm) | Judgment result | Inhibitory zone diam (mm) | Judgment result | ||
Cefoperazone | 75 μg | 0 | R | 22.8 ± 1.2 | S | 0 | R | 22.3 ± 1.5 | S | 0 | R | 24.8 ± 0.6 | S | 0 | R | 20.5 ± 0.9 | S | 27.9 ± 0.5 | S | 23.1 ± 0.5 | S | 31.5 ± 0.5 | S | 20.5 ± 0.9 | S | 30 ± 1.2 | S |
Polymyxin B | 300 IU | 14.9 ± 0.2 | S | 13.9 ± 1.1 | S | 17.8 ± 0.4 | S | 15.9 ± 0.4 | S | 14.9 ± 0.6 | S | 15.9 ± 0.4 | S | 13.9 ± 0.5 | S | 13 ± 0.7 | S | 14.3 ± 1.0 | S | 11 ± 0.4 | I | 16 ± 0.4 | S | 10 ± 0.5 | I | 13 ± 1.0 | S |
Ofloxacin | 5 μg | 29.9 ± 0.7 | S | 32.2 ± 0.4 | S | 34.7 ± 1.0 | S | 34.1 ± 1.5 | S | 30.7 ± 0.5 | S | 31.9 ± 0.7 | S | 29.9 ± 2.4 | S | 28.8 ± 1.1 | S | 29.7 ± 0.4 | S | 29.2 ± 0.7 | S | 33 ± 2.0 | S | 40 ± 1.0 | S | 0 | R |
Amikacin | 30 μg | 0 | R | 22.0 ± 0.6 | S | 0 | R | 20.2 ± 1.6 | S | 0 | R | 21.8 ± 2.0 | S | 0 | R | 22.2 ± 0.8 | S | 27.3 ± 0.6 | S | 26.1 ± 1.0 | S | 37 ± 1.0 | S | 32.5 ± 0.6 | S | 26 ± 1.0 | S |
Zone diameter data represent means ± standard deviations from three independent experiments performed in triplicate. R, resistant; S, susceptible; I, intermediate.