TABLE 5.

Growth of control and resistant strains in unsupplemented medium and in medium containing tachyplesin I^a

Strain	Growth parameter	Value with indicated medium
		MHB		MHB plus tachyplesin I
		Control strain	Resistant strain	Control strain	Resistant strain
E. coli ATCC 25922-96	K	0.995	0.998	1.391	1.15
	Vmax	0.25	0.169	0.225	0.195
	Lag time (h)	1	2	21	9
E. coli F41-80	K	1.456	1.474		1.017
	Vmax	0.207	0.198		0.165
	Lag time (h)	1	8	36	10
A. hydrophila XS91-4-1-69	K	1.337	1.411	1.216	1.225
	Vmax	0.197	0.229	0.179	0.165
	Lag time (h)	1	2	16	8
P. aeruginosa CGMCC1.2620-80	K	1.422	1.436	1.123	1.094
	Vmax	0.346	0.262	0.184	0.272
	Lag time (h)	2	7	19	10

^aStrains were grown in unsupplemented medium for 26 h or in medium containing half the MIC of tachyplesin I for the corresponding control strains for 40 h. We measured the OD₆₀₀ values every hour. K, maximum absorbance; V_max, rate of increase in absorbance (h⁻¹). Values indicate overall means from two replicates using all strains.