FIG 1.
Greatly different sensitivities of rabbit, mouse, and human PMNs to S. aureus leukocidins. PMNs purified from rabbit, mouse, and human whole blood were coincubated with leukocidins in the indicated dose range for 4 hours, followed by parallel measurement of cellular ATP content (blue curves and EC50 values) and LDH release (red curves and EC50 values) as indicators for cell viability or pore formation, respectively. n.d., no EC50 value determined. Error bars represent SEM from at least 2 independent experiments for each toxin.