FIG 2.

Decoration of HHQ exhibits variable biofilm activity against C. albicans. (a) A panel of HHQ-derivatized analogues were incubated with filamentous C. albicans and screened for biofilm formation using the metabolic XTT biofilm assay. The data are presented as OD₄₉₂ spectrophotometric output normalized to the untreated control and are representative of at least three independent biological replicates, with error bars representing SEM. (b) Dose-dependent XTT analysis of selected antibiofilm compounds applied at 10, 50, and 100 μM. The data are the averages from at least two independent biological replicates, each constituting eight technical replicates. Statistical analysis of both data sets was performed by one-way analysis of variance (ANOVA) with Bonferroni corrective testing, and the results are presented relative to the MeOH control values; *, P ≤ 0.05; **, P ≤ 0.01; ***, P ≤ 0.001.