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. 2016 Aug 10;12(4):2912–2917. doi: 10.3892/ol.2016.4994

Figure 1.

Figure 1.

(A) Structure of GSL from Siegesbeckia glabrescens. (B) Effect of GSL on Shh-CM-induced ALP activity. C3H10T1/2 cells were treated with GSL and/or Shh-CM for 96 h and ALP activity was measured. Cyclopamine (5 µM) was used as the positive control. . (C) Effect of GSL on Gli1-mediated transcriptional activity. C3H10T1/2-Gli1-luc cells were seeded onto a 96-well plate and were subsequently induced with Shh-CM. At the same time, various concentrations (0,5,10 and 20 µM) of GSL were added for 30 h. Cyclopamine (5 µM) was used as the positive control. The ratio of luciferase reporter activity of the GSL-treated cells/vehicle-treated cells was calculated as the inhibitory effect on Shh-CM-stimulated Gli1-mediated transcriptional activity. *P<0.05 vs. Shh-CM. GSL, germacranolide sesquiterpene lactone; Shh-CM, Sonic Hedgehog conditioned medium; ALP, alkaline phosphatase; Gli1, glioma-associated oncogene homolog 1.