Table I.
Primer sequences for the construction of the mutant Brucella melitensis strain 16MΔomp31.
| Target | Primer | Primer sequences (5′→3′) | Restriction site |
|---|---|---|---|
| Upstream | OMP31-N | F: GCATGCCCCATCCTGTCGCTTTGTGT | SphI |
| R: CTCGAGAATCACATTCGGCGAAAAAG | XhoI | ||
| Downstream | OMP31-C | F: CTCGAGTTATCAGGCGGCGGGAATTG | XhoI |
| R: GGATCCAGGCTTCGCTCGGTCACAGG | BamHI | ||
| SacB gene | sacB | F: GGATCCGGGCTGGAAGAAGCAGACCGCTA | BamHI |
| R: GAGCTCGCTTATTGTTAACTGTTAATTGTCC | SacI | ||
| Detection for Brucella | L7/L12 | F: ATGGCTGATCTCGCAAAGA | – |
| R: TTACTTGAGTTCAACCTTG | – | ||
| Detection for mutant | DC | F: CGTACATATTGGCGAGGGTG | – |
| R: CCGTCAGGAAGGGTTCAGTG | – |
omp31, outer membrane protein 31.