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Primer sequences for detection of Bax, Bcl-2 and TNF-α by reverse transcription-quantitative polymerase chain reaction.
| Primers | Primer sequences (5′→3′) |
|---|---|
| GAPDH | F: GCAGTGGCAAAGTGGAGATT |
| R: CCTTGACTGTGCCGTTGAAT | |
| TNF-α | F: GCCTCCCTCTCATCAGTTCTAT |
| R: CACTTGGTGGTTTGCTACGAC | |
| Bax | F: GCCTTTTTGCTACAGGGTTT |
| R: TGCTGTCCAGTTCATCTCCA | |
| Bcl-2 | F: GACTTCTCTCGTCGCTACCG |
| R: ACAATCCTCCCCCAGTTCAC |
GAPDH, glyceraldehyde-3-phosphate dehydrogenase; TNF, tumor necrosis factor; Bax,; Bcl-2,; F, forward; R, reverse.
