Table II.
Quantitative polymerase chain reaction components.
| Content | Volume (µl) | Concentration |
|---|---|---|
| cDNA | 1 | – |
| Forward primer | 0.5 | 20 pmol/µl |
| Reverse primer | 0.5 | 20 pmol/µl |
| 2X Mix | 12.5 | 1X |
| 10X SYBR Green I | 1 | 0.4X |
| ddH2O | 9.5 | – |
| Total volume | 25 | – |
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