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Methylation specific-polymerase chain reaction primers.
| Gene | Forward primer 5′-3′ | Reverse primer 5′-3′ |
|---|---|---|
| MMP-14 (U) | TTTATTGAAGATAAACGTGTTTTGA | CATAAACAAAAAAAACAAAAAACAACA |
| MMP-14 (M) | TTTATCGAAGATAAAGGCGTTTC | GTAAACAAAAAAAACAAAAAACAACG |
| TGFβ-1 (U) | GTGGGTTTTTATTATTAGTATGTGG | AAATCCTATCCAAACTACAACTCAC |
| TGFβ-1 (M) | TTGTGGGTTTTTATTATTAGTACGC | AAATCCTATCCAAACTACGACTCG |
U, unmethylated; M, methylated; MMP, matrix metalloproteinase; TGF, transforming growth factor.
