Skip to main content
. 2016 Aug 4;12(4):2027–2038. doi: 10.3892/etm.2016.3570

Figure 4.

Figure 4.

Bisoprolol attenuates cardiac fibrosis in response to pressure overload in vivo. (A) Picrosirius red staining of interstitial (top) and perivascular (bottom) regions of left ventricles of C57BL/6J mice following treatment of saline or bisoprolol at 8 weeks after sham or aortic banding (AB) surgery. Quantification of collagen volume fraction from (B) interstitial and (C) perivascular regions (n=4–6 per group). (D) Representative western blots of connective tissue growth factor (CTGF), tissue growth factor (TGF)-β1 and collagen 1a at 8 weeks after sham or AB surgery. (E) Quantitative mRNA expression levels of CTGF, TGF-β1 and collagen 1a in the groups (n=3 per group). Values represent the mean ± standard error of the mean. *P<0.05 vs. all other groups. **P<0.05 vs. M-Biso and H-Biso AB groups; ***P<0.05 vs. H-Biso AB groups. Biso, bisoprolol; L, low-dose; M, middle-dose; H, high-dose; GAPDH, glyceraldehyde 3-phosphate dehydrogenase.