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. 2016 Aug 11;12(4):2301–2306. doi: 10.3892/ol.2016.5002

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Copyright: © Qiu et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 2. — miR-186 upregulation promoted melanoma cell proliferation. (A) Validation of miR-186 expression levels by polymerase chain reaction analysis subsequent to transfection. (B) MTT assays revealed that upregulation of miR-186 promoted the growth of SKMEL-28 cells and miR-186-in inhibited the growth of SKMEL-28 cells. (C) Representative micrographs (left) and quantification (right) of crystal violet staining of the cell colonies. (D) Cell cycle distribution in SKMEL-28 cells with miR-186, miR-186-in or miR-NC transfection. *P<0.05 versus control. miR-186, microRNA-186; in, inhibitor; NC, negative control; PCR, polymerase chain reaction; MTT, methyl thiazolyl tetrazolium.