Figure 4.
Analysis of SP cells in (A) PLC/RAF/5 (control) and (B) 5-Fu-treated PLC/RAF/5 cells by flow cytometry analysis. Cells were stained with Hoechst 33342 in the absence or presence of verapamil. The SP cells were gated and represented as a percentage of the total population. Flow cytometry analyses were performed in triplicate. 5-Fu, 5-fluorouracil; SP, side population.