Table IV.

Gene validation using RT-qPCR (n=3).

		Microarray		RT-qPCR
Gene	Molecular function	Fold-change (mean ± SD)	P-value	Fold-change (mean ± SD)	P-value
MYLK	Actin binding, cytoskeletal protein binding, calmodulin binding, protein kinase activity, magnesium ion binding, purine ribonucleotide binding, protein serine/threonine kinase activity, calcium ion binding, adenyl nucleotide binding, ribonucleotide binding	−2.14±0.165	0.005	0.478±1.708	0.028
MYH11	Actin binding, cytoskeletal protein binding, calmodulin binding, structural constituent of muscle, protein kinase activity, magnesium ion binding, adenyl nucleotide binding, ribonucleotide binding	−3.7±0.201	0.015	0.476±0.089	0.017
FLNA	Actin binding, cytoskeletal protein binding	−2.11±0.246	0.031	0.945±0.151	0.053
SOD3	Carbohydrate binding, copper ion binding, polysaccharide binding, pattern binding, heparin binding, polysaccharide binding	−2.08±0.150	0.003	0.380±0.032	0.001
TAGLN	Actin binding, cytoskeletal protein binding	−2.43±0.178	0.008	0.386±0.020	0.005
PKD-1	Protein complex binding, sugar binding	−2.48±0.145	0.002	0.469±0.151	0.022

RT-qPCR, reverse transcription-quantitative polymerase chain reaction; SD, standard deviation; MYLK, myosin light chain kinase; MYH11, myosin heavy chain 11; FLNA, filamin A; SOD3, superoxide dismutase 3, extracellular; TAGLN, transgelin; PKD-1, polycystin 1, transient receptor potential channel interacting.