Figure 3.

The effect of miR-101 and COX-2 siRNA on proliferation and migration ability of glioma cells. (A and B) Viability of wild type glioma cells and cells transfected with miR-101, COX-2 siRNA or empty vectors was determined by Cell Counting Kit-8 assays. Viability was set at 100% on day 1. (C-E) Migration ability of WT glioma cells and cells transfected with the miR-101, COX-2 siRNA or empty vectors was determined by Transwell assays. The (C) microscope images (magnification, ×4000) and (D and E) statistical analysis indicate that the transfection of either miR-101 or COX-2 siRNA significantly suppresses the cell migration rate in the two glioma cell lines. Data are expressed as the mean ± standard deviation (n=3), **P<0.01 vs. control (wild-type U373/U87 cells). siRNA, small interfering RNA; COX-2, cyclooxygenase-2; WT, wild type; NC, empty vector.