Figure 3.

Effects of ESRP1 overexpression on the development of bone metastases of MDA-MB-231 cells. (A) Representative histological pictures of bone metastases (hematoxylin and eosin staining; scale bar, 1 mm). (B) Histomorphometric analysis of the tumor burden in bone. Data are expressed as tumor area (mm²; n=5/group). (C) Immunohistochemical detection of the expression of panCD44 and CD44v9 in cancer cells colonized in bone (asterisk, bone; scale bar, 100 µm). CD, cluster of differentiation; CD44v, variant isoform of CD44; ESRP1, epithelial splicing regulatory protein 1; EV, empty vector; Cl, clone; T, tumor; BM, bone marrow; n.s., not significant.