Figure 6.

Effects of CD44v8-10 overexpression on the development of bone metastases of MDA-MB-231 cells. (A) Representative histological pictures of bone metastases (hematoxylin and eosin staining; scale bar, 1 mm). (B) Histomorphometric analysis of the tumor burden in bone. Data are expressed as tumor area (mm²; n=5/group). (C) Immunohistochemical detection of panCD44 and CD44v9 expression in cancer cells colonized in bone (asterisk, bone; scale bar, 100 µm). CD, cluster of differentiation; CD44v, variant isoform of CD44; EV, empty vector; Cl, clone; T, tumor; BM, bone marrow; n.s., not significant.