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. Author manuscript; available in PMC: 2017 Feb 1.
Published in final edited form as: Nat Methods. 2016 Jul 11;13(8):665–672. doi: 10.1038/nmeth.3921

Figure 3. Survival of mammalian cells during repeated cryo-arrest cycles.

Figure 3

(a) General scheme of an imaging experiment using three consecutive cycles of reversible cryo-arrest. (b) Effect of three consecutive cycles of cryo-arrest on cell survival and morphology. Representative images of HeLa, Cos7 and MDCK cells transfected with eGFP. After each cycle cells were stained with propidium iodide (PI) to mark dead cells. In the last row an overlay of eGFP (green) and PI staining (red) after the last cycle is shown. Scale bar: 20 µm. (c) Quantification of the fraction of PI-negative cells during the course of the three consecutive cycles of cryo-arrest for the indicated cell lines (Cos7 n=64, HeLa n=70, HCT116 n=100, MCF7 n=77 and MDCK n=73 cells). All data is shown as mean ± s.d. from 3 independent experiments.