Figure 2.

Caspases activities and cyto c release following exposure to V8. (A-C) Human multiple myeloma RPMI 8226 cells were treated with various concentrations of V8 for 48 h and the activation of caspase-3, −8 and −9 was detected by fluorometric assay. (D) Western blotting was performed to determine the level of cyto c in the mitochondria and cytoplasm of RPMI 8226 cells. (E and F) Densitometry showing the ratio of cyto c relative to (E) COX4 in mitochondria and (F) β-tubulin in the cytoplasm for each V8 concentration. Data are presented as the mean ± standard error of the mean of three independent experiments. *P<0.05, **P<0.01 vs. untreated cells. V8, 7-{4-[Bis-(2-hydroxyethyl)-amino]-butoxy}-5-hydroxy-8-methoxy-2-phenylchromen-4-one; cyto c, cytochrome c; COX4, cytochrome c oxidase subunit IV.