Table I.

Primer sequences for reverse transcription-polymerase chain reaction.

Gene	Primers	Product size (bp)
CD45	F: 5′-TGTCGTGACGATCAATGACCA-3′	306
	R: 5′-ACATCCACTTTGCCCTCTGCT-3′
CD133	F: 5′-ACCAGTTGCCTGACGGAAATC-3′	304
	R: 5′-GCGTACAAGACCCATTGCAGAT-3′
CD117	F: 5′-GGCATCACCATCAAAAACGTG-3′	317
	R: 5′-TGGCGTTCGTAATTGAAGTCG-3′
CD326	F: 5′-GATGAAGGCGGAGATGACTCAC-3′	315
	R: 5′-CGAGATGCAAATGTGTCCTGAA-3′
CK18	F: 5′-GGATGCTCCCAAATCTCAGGA-3′	331
	R: 5′-TGATTCCAGATGCAGAAGGACC-3′
CK19	F: 5′-TCCACACCAGGCATTGACCTA-3′	403
	R: 5′-GCCTCGACTTGATGTCCATGA-3′
CLDN3	F: 5′-CGAGCTCTCATCGTGGTGTCTA-3′	256
	R: 5′-CGTACAACCCAGTTCCCATCTC-3′
c-Met	F: 5′-TCAACAGCGGCAATTCTAGACA-3′	364
	R: 5′-GTGGTGCAGCAGATGATCTCTG-3′
Cx43	F: 5′-TTCATGCTGGTGGTGTCCTTG-3′	323
	R: 5′-CGATTTTGCTCTGCGCTGTAGT-3′
HNF3β	F: 5′-CCAACAAGATGCTGACGCTGA-3′	331
	R: 5′-TGAACCTGAGAAGCCTGTGTCC-3′
HNF4α	F: 5′-AGGCAGTGCGTGGTAGACAAA-3′	421
	R: 5′-TGAACACCATGGACCTCTTGG-3′
OV-6	F: 5′-ATGACATGAAGGTTGTCCTCGG-3′	263
	R: 5′-CCCCTTGGTCTTAACATGCTGA-3′
Sca-1	F: 5′-GCACGATGATCCCATTTGGT-3′	230
	R: 5′-TGCTGCGTTGCAAAGATCTG-3′
Thy1	F: 5′-TGCCGTCATGAGAATAACACCA-3′	201
	R: 5′-ACACATGTAGTCGCCCTCATCC-3′
SHH	F: 5′-AACTCACCCCCAATTACAACCC-3′	321
	R: 5′-GGATGCGAGCTTTGGATTCA-3′
IHH	F: 5′-ACCGCGACCGAAATAAGTACG-3′	301
	R: 5′-AAAGCTCTCAGCCTGTTTGGC-3′
PTCH	F: 5′-TGTTGGTGTGGACGACGTCTT-3′	354
	R: 5′-GGTTCAACTTGAATCACCCGG-3′
VIM	F: 5′-TACATCGACAAGGTGCGCTTC-3′	422
	R: 5′-TCGATCTGGACATGCTGTTCC-3′
α-SMA	F: 5′-TGGAGAAGAGCTACGAACTGCC-3′	342
	R: 5′-ATAGAGAAGCCAGGATGGAGCC-3′

F, forward; R, reverse; CK, cytokeratin; CLDN3, claudin-3; Cx43, connexin 43; HNF, hepatocyte nuclear factor; OV-6, oval cell marker 6; Sca-1, stem cell antigen 1; Thy1, thymocyte antigen 1; SHH, sonic hedgehog; IHH, Indian hedgehog; PTCH, patched; VIM, vimentin; α-SMA, α-smooth muscle actin.