Table III.

Sequences of the primers and probes used for quantitative polymerase chain reaction.

Gene	Primers and probes	Product size (bp)
IHH	F: 5′-CGGCCATCACTCAGAGGAAT-3′	104
	R: 5′-CTGCTAAGCGCGCCAGTAGT-3′
	P: 5′-FAM-TTTACACTATGAGGGCCGCGC-TAMRA-3′
PTCH	F: 5′-GCCTTTCTGACAGCCATTGG-3′	103
	R: 5′-CACCCAGCAGAGTGGACACA-3′
	P: 5′-FAM-CAAGAACCACAGGGCTATGCTCGC-TAMRA-3′
GLI1	F: 5′-CCTGAAGTGGGCAGGTTAGG-3′	100
	R: 5′-GCTGAGTGTTGTCCAGGTCAAG-3′
	P: 5′-FAM-AGGGCAGGTGTGTAACCCTCTGG-TAMRA-3′

IHH, Indian hedgehog; PTCH, patched; GLI1, glioma-associated oncogene 1; F, forward; R, reverse; P, probe.