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. 2016 Sep 27;11(9):e0163342. doi: 10.1371/journal.pone.0163342

Fig 5. Combined ethanol and cholesterol feeding induces liver fibrosis and increases Type I collagen and hydroxyproline in the liver.

Fig 5

Mice were fed CTR, Chol, EtOH and EtOH+Chol diets for 3 months. A-E, Mason’s Trichrome staining. F-J, second harmonic generation (SHG) microscopy. K, representative immunoblots of Type I collagen (Collagen-I) and actin. L, quantification of Type I collagen immunoblots by densitometry. M, hydroxyproline in the liver. Values are means ± S.E.M. a, p < 0.05 vs CTR; b, p < 0.05 vs Chol; c, p < 0.05 vs EtOH (n = 4 per group).