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. 2016 Sep 27;11(9):e0163342. doi: 10.1371/journal.pone.0163342

Fig 6. Combined ethanol and cholesterol feeding stimulates stellate cell activation and alters TGF-β signaling in the liver.

Fig 6

Mice were fed CTR, Chol, EtOH and EtOH+Chol diets for 3 months. A, representative immunoblots of α-SMA, TGF-β1, BAMBI, Smad2/3, phospho-Smad2/3 and actin. B, quantification of α-SMA immunoblots by densitometry. C, quantification of TGF-β1 immunoblots. D, quantification of BAMBI immunoblots. E, detection of miR33a by RT-qPCR. F, quantification of phospho-Smad2/3 immunoblots. Values are means ± S.E.M. a, p < 0.05 vs CTR; b, p < 0.05 vs Chol; c, p < 0.05 vs EtOH (n = 4 per group).