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. 2016 Sep 27;12(9):e1005827. doi: 10.1371/journal.ppat.1005827

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Fig 4 — A. Y2H assay showing that PpEC23 interacts through the C-terminal CM. SD/X/-LWH, SD/-LWHA and SD/-LW represent SD/X-α-gal (-Leu/-Trp/-His), SD (-Leu/-Trp/-His/-Ade) and SD (-Leu/-Trp), respectively. The structural diagrams of PpEC23_ns or truncated constructs of PpEC23 are provided next to the relevant strain. The interaction of murine p53 (p53) and SV40 large T-antigen (T) was used as a positive control for the system, and human lamin C (lam) was used as a negative control. B. BiFC assay showing that PpEC23 interacts with itself in planta. YFP, yellow fluorescent protein epifluorescence. BF, bright field. DAPI signal was used as a nuclear marker. Arrows indicate nuclei. Representative images are shown (n ≥ 20). Bar = 20 μm.