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. 2016 Sep 27;12(9):e1005827. doi: 10.1371/journal.ppat.1005827

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Fig 5 — A. PpEC23 and GmSPL12l interaction confirmed by Y2H. The empty vector, pGADT7, was included as a negative control. SD/-LWHA and SD/-LW represents SD (-Leu/-Trp/-His/-Ade) and SD (-Leu/-Trp), respectively. The structural diagrams of PpEC23_ns or truncated constructs of PpEC23 are shown next to the corresponding strain. B. PpEC23 and GmSPL12l interaction detected in N. benthamiana nuclei by BiFC assay. YFP/GFP, yellow or green fluorescent protein epifluorescence. BF, bright field. DAPI signal was used as a nuclear marker. Arrows indicate nuclei. Representative images are shown (n ≥ 20). Bar = 20 μm. C. PpEC23 and GmSPL12l interaction confirmed by co-immunoprecipitation assay (CoIP). MW, molecular weight marker. The solid triangle indicates the band corresponding to the GFP-GmSPL12l fusion protein. The white triangle indicates the GFP protein band. @FLAG and @GFP indicate detection using anti-FLAG and anti-GFP antibodies, respectively.