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. 2016 Sep 27;12(9):e1005827. doi: 10.1371/journal.ppat.1005827

Fig 7. GmSPL12l is a negative regulator of plant immunity.

Fig 7

A. Phenotypes of soybean plants 21 dpi with BPMV. A. Soybean plants infected with the BPMV empty vector (BPMV:00) (left) and BPMV:GmSPL12li (right). Bar = 5 cm. Representative images are shown (n ≥ 6). B. Heights of plants at 21 dpi with BPMV:00 (white bars) and BPMV:GmSPL12li (black bars). A t-test was performed for the pair-wise comparison. The * indicates significant difference (P < 0.05). Six biological replicates were performed. C. Fold change of GmSPL12l (left panel) and GmPR1a (right panel) mRNA in BPMV:00 (white bars) and BPMV:GmSPL12li (black bars) plants. GmAct1 was used as the internal reference gene. A t-test was performed for each comparison. The * indicates significant difference (P < 0.05). Four biological and four technical replicates were performed. D. Representative leaves from BPMV:00 (left) and BPMV:GmSPL12li (right) plants (n ≥ 18). Bar = 1 cm. E. Downy mildew symptoms on leaves of BPMV:00 (left) and BPMV:GmSPL12li (right) plants inoculated with P. manshurica. Leaves were photographed at 28 days post BPMV inoculation (7 days post P. manshurica inoculation). Representative third trifoliate leaves are shown (n ≥ 12). The assay was repeated three times. Bar = 1 cm.