Table 2. Strains and plasmids.

Strain or plasmid	Genotype or relevant phenotype *	Source or reference
E. coli
DH5α	F- endA1 glnV44 thi-1 recA1 relA1 gyrA96 deoR nupG Φ80dlacZΔM15 Δ(lacZYA-argF)U169 hsdR17(rK - mK +) λ–	Invitrogen
TOP10	F- mcrA Δ(mrr-hsdRMS-mcrBC) Φ80lacZΔM15 ΔlacX74 nupG recA1 araD139 Δ(ara-leu)7697 galE15 galK16 rpsL(StrR) endA1 λ-	Invitrogen
P. syringae pv. tomato
DC3000	Wild type, Rifr
CUCPB5115	∆CEL::ΩSp/Smr, Rifr Spr	[72]
P. fluorescens
EtHAn	P. fluorescens Pf0-1 carrying a working TTSS from P. syringae pv. Syringae, Cmr	[33]
Agrobacterium tumefaciens
GV3101	Carries Vir plasmid encoding T-DNA transfer machinery, Rifr, Gmr
Saccharomyces cerevisiae
EGY48	MATa, his3, trp1, ura3, LexA op(x6) -LEU2	Clontech
AH109	MATa, trp1-901, leu2-3, 112, ura3-52, his3-200, gal4∆, gal80∆, LYS2::GAL1 UAS -GAL1 TATA -HIS3, GAL2 UAS -GAL2 TATA -ADE2,URA3::MEL1 UAS -MEL1 TATA -lacZ	Clontech
YTK12	suc2Δ9 trp1Δ ade2-101 ura3-52	[42]
Plasmids
pCR8⁄GW⁄TOPO	Gateway-compatible entry vector, Spr	Invitrogen
pEDV6	Gateway-compatible version of pEDV3, Gmr	[29]
pSITEII-3C1	Gateway-compatible binary vector for transiently over-expression of EGFP-fused protein in planta, Smr	[73]
pBI121	Binary vector for transformation in plant, Kmr in bacteria, Kmr in plants	[74]
pSuc2t7M13ori	Yeast signal sequence trap vector, Ampr in bacteria, -Trp in yeast	[42]
pLexA	Bait plasmid for yeast LexA two-hybrid system containing BD domain, Ampr in bacteria, -His in yeast	Clontech
pB42AD	Library plasmid for yeast LexA two-hybrid system containing AD domain, Ampr in bacteria, -Trp in yeast	Clontech
P8op-lacZ	LacZ reporter plasmid for yeast LexA two-hybrid system, Ampr in bacteria, -Ura in yeast	Clontech
pGBKT7	Protein expression plasmid for yeast Gal4 two-hybrid system containing BD domain, Kmr in bacteria, -Trp in yeast	Clontech
pGADT7	Protein expression plasmid for yeast Gal4 two-hybrid system containing AD domain, Ampr in bacteria, -Leu in yeast	Clontech
pSPYNE-35S	BiFC plasmid containing YFP N-terminal fragment fused to the C-terminus of insertion, Kmr in bacteria, Kmr in plants	[47]
pSPYCE-35S	BiFC plasmid containing YFP C-terminal fragment fused to the C-terminus of insertion, Kmr in bacteria, Barr in plants	[47]
phygII-SPYNE(R)155	BiFC plasmid containing eYFP N-terminal fragment fused to the N-terminus of insertion, Kmr in bacteria, Hygr in plants	[75]
pkanII-VYCE(R)	BiFC plasmid containing Venus C-terminal fragment fused to the N-terminus of insertion, Kmr in bacteria, Kmr in plants	[75]
pBPMV-IA-V2	BPMV-based gene silencing vector, Ampr	[50]
pYL192	TRV-based gene silencing vector, containing TRV RNA1, Kmr in bacteria, Kmr in plants	[52]
pYL279	TRV-based gene silencing vector, containing TRV RNA2-Gateway cassette, Kmr in bacteria, Kmr in plants	[53]
pYL124	TRV-based gene silencing positive control, containing TRV RNA2-NtPDS, Kmr in bacteria, Kmr in plants	[52]

*: Antibiotics concentrations (μg/ml) were used as follows: Rifampicin (Rif) 100, Kanamycin (Km) 75, Gentamycin (Gm) 50, Spectinomycin (Sp) 50, Chloramphenicol (Cm) 30, Ampicillin (Amp) 100, Streptomycin (Sm) 100, Hygromycin B (Hyg) 30 and Basta (Bas) 100.