Table 3. Proportion of bacteria recovered from Leptospermum scoparium that showed siderophore production activity on CAS agar and antagonist effect against Ilyonectria lirodendri and Neofusicoccum luteum.
Origin site | Siderophore production | Antagonistic against Ilyonectria liriodendri | Antagonistic against Neofusicoccum luteum | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
- | + | ++ | +++ | - | + | ++ | +++ | - | + | ++ | +++ | |
Island Hills Station | 63.5 | 9.5 | 4.8 | 22.2 b | 85.8 | 6.3 | 6.3 | 1.6 a | 90.5 | 6.3 | 1.6 | 1.6 b |
Travis Wetland | 38.6 | 10.0 | 14.3 | 37.1 a | 85.7 | 12.9 | 1.4 | 0.0 a | 85.7 | 11.4 | 2.9 | 0.0 b |
West Coast | 23.7 | 13.6 | 32.2 | 30.5 a | 76.3 | 20.3 | 0.0 | 3.4 a | 61.0 | 15.3 | 6.7 | 17.0 a |
The different letter on the last column indicates significantly different activity responses among the sites at p ≤0.05 according Mann-Whitney test. For siderophore production, (-): no clear zone, (+): clear zone less than 5 mm, (++): clear zone 10 mm > x > 5 mm diameter, and (+++): clear zone ≥ 10 mm in diameter; For antagonist effect, (-): no inhibition activity, (+): weak activity–fungal colony showing small indent around bacterial colony but mycelial growth still to the plate edge without visible inhibition zone, (++): moderate activity–fungal growth up to bacterial colony but restricted with inhibition zone < 1mm, (+++): strong activity–fungal growth restricted with inhibition zone > 1mm