Fig. 6.

Aftin-5 modifies extracellular but not intracellular Aβ₃₈/Aβ₄₀/Aβ₄₂ ratios. Cells were exposed to 100 μM Aftin-5 for 18 h, in the absence or presence of 1 μM DAPT or 1 μM ‘Torrey Pines’ compound. Cell supernatant (A) and cell pellets (B) were recovered and Aβ₃₈, Aβ₄₀, and Aβ₄₂ amyloid levels were measured by an ELISA assay. Amyloid levels are expressed as fold change over their concentrations in control, non-treated cells. Basal extracellular (A) and intracellular (B) levels of amyloids were, respectively, 61.9 and 83.5 pg/mL (Aβ₃₈), 471 and 48.9 pg/mL (Aβ₄₀), 28.9 and 33.6 pg/mL (Aβ₄₂). Representative of two independent experiments, errors bars represent standard deviation of triplicate values.