Table 1.
Data collection and refinement statistics.
| Data Set | G505-4w006native | G505-4w006Ta peak | G505-4w006SeMet peak |
|---|---|---|---|
| Diffraction Data Statistics | |||
| Synchrotron Beamline |
SLS PXII |
SLS PXII |
SLS PXII |
| Wavelength |
0.99995 |
1.25473 |
0.97894 |
| Resolution (Å) |
75-3.0 |
75.4-3.8 |
43.6-4.4 |
| Space Group |
P 32 |
P 32 |
P 32 |
| Cell dimensions |
|
|
|
| a (Å) |
86.94 |
87.06 |
87.11 |
| b (Å) |
86.94 |
87.06 |
87.11 |
| c (Å) |
200.72 |
201.30 |
199.69 |
| α (°) |
90 |
90 |
90 |
| β (°) |
90 |
90 |
90 |
| γ (°) |
120 |
120 |
120 |
| I/σI* |
18.4 (1.9) |
27.49 (5.83) |
16.58 (3.82) |
| Completeness (%)* |
99.8 (98.8) |
99.6 (97.3) |
97.3 (83.3) |
| Redundancy* |
6.3 |
10.2 |
5.1 |
| Rsym (%)* |
6.2 (90.7) |
7.7 (42.2) |
6.9 (43.4) |
| Refinement Statistics | |||
| Resolution (Å) |
75-3.0 |
|
|
| Number of Reflections |
33933 |
|
|
| Rwork/Rfree |
0.199/0.241 |
|
|
| Number of Atoms |
|
|
|
| Protein |
6298 |
|
|
| DNA |
1589 |
|
|
| Water/Solutes |
27 |
|
|
| B-factors |
|
|
|
| Protein |
123.4 |
|
|
| DNA |
150.2 |
|
|
| Water/Solutes |
92.6 |
|
|
| R.M.S Deviations |
|
|
|
| Bond lengths (Å) |
0.010 |
|
|
| Bond Angles (°) |
0.623 |
|
|
| Ramachandran Plot |
|
|
|
| Preferred |
753 (97.9 %) |
|
|
| Allowed |
16 (2.1%) |
|
|
*Values for the highest resolution shell are shown in parenthesis.