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. 2016 Sep 22;167(1):219–232.e14. doi: 10.1016/j.cell.2016.09.006

Figure S3.

Figure S3

Activity of the Triple ETR Combination in Multiple Cell Types, Related to Figure 3

(A) Time-course flow cytometry analysis of LVTetO7/GFP K-562 cells upon transfection with mRNAs encoding for the indicated ETRs. Data show percentage of eGFP-negative cells (mean ± SEM; n = 3 independent transfections for each treatment condition).

(B) Time-course flow cytometry analysis of AAVS1GFP/TetO7 B-lymphoblastoid cells upon transfection with mRNAs encoding for the indicated ETRs. Data show percentage of eGFP-negative cells (mean ± SEM; n = 3 independent transfections for each treatment condition).

(C) Time-course flow cytometry analysis of LVTetO7/GFP NIH 3T3 cells upon transfection with mRNAs encoding for the indicated ETRs. Data show percentage of eGFP-negative cells (mean value of 2 independent transfections for each treatment condition).

(D) Fold change in the expression level of DNMT3L over HPRT1 in K-562 cells (n = 3), B-lymphoblastoid cells (n = 3), HEK-293T cells (n = 3), human primary T Lymphocytes (n = 3), human induced Pluripotent Stem Cells (iPSC) (n = 7) and H9 human ES cell line (n = 1).