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. 2016 Sep 22;167(1):219–232.e14. doi: 10.1016/j.cell.2016.09.006

Figure S7.

Figure S7

Specificity Analyses of the Triple ETR Combination, Related to Figure 7

(A) Histogram showing the percentage of tdTomato-negative cells at 25 days post-transfection with plasmids encoding for the indicated TALE- and dCas9- based ETRs with or without the wild-type DNMT3L (wt.D3L). Data are shown as mean ± SEM of 3 independent transfections for each treatment condition.

(B) Histogram showing the fold change in the expression levels of the indicated genes at day 7 upon transient transfection of plasmids encoding for TALE:K targeting the indicated genes. Data are represented as mean ± SEM of 3 independent transfections for each treatment condition.

(C) Enrichment of DMRs (defined at low stringency cutoff: nominal p value < 0.01, logFC > 1) in repetitive elements, defined by RepeatMasker annotation from UCSC Genome Browser track. For each experiment, the ratio of selected DMRs overlapping a specific class of repetitive element was calculated. Horizontal black bars represent the ratio of all analyzable methylated regions (n = 198886) over repetitive elements. None of the ratios was found significantly higher than the expected (test: chi-square).