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. 2016 Sep 28;7:1556. doi: 10.3389/fmicb.2016.01556

FIGURE 2.

FIGURE 2

pICV8 integration products. (A) Diagram showing primers used to demonstrate cassette insertions in pICV8. (B,C) Amplicons derived from pICV8 cassette insertion products in strains 6479 and 3430-1, respectively. Lanes 1–3 were amplified using 4E-L2 and 8E-U1. Lanes 4–6 were amplified using 4E-L2 and drfA12-U1. Lanes 7–9 were amplified using aadA2-L2 and 8E-U1. Lanes 1, 4, and 7, strains grown without streptomycin. Lanes 2, 5, and 8, trains grown with 32 μg/mL streptomycin. Lanes 3, 6, and 9, strains grown with 256 μg/mL streptomycin. Lanes 10, molecular weight 1 Kb plus (Invitrogen, Carlsbad, CA, USA).