FIGURE 2.

Effect of M3 or 3m1 mutation of PIN1 on seedling development. (A) Six-day-old seedlings showing cotyledon defects by PIN1 mutations. Bar = 0.5 mm for all. (B) Quantification of cotyledon defects shown in (A). All the segregated F1 progeny seedlings of self-fertilized heterozygous pin1 (±) mutant background were observed for pin1 and pin1 with PIN1 (ProPIN1:PIN1:GFP), M3PIN1 (M3, ProPIN1:M3PIN1:GFP), and 3m1PIN1 (3m1, ProPIN1:3m1PIN1:GFP). Total 403–485 seedlings from 7 to 8 independent lines per construct were observed. cot, cotyledon; WT, wild-type.