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. 2016 Sep 28;7:1479. doi: 10.3389/fpls.2016.01479

FIGURE 5.

FIGURE 5

Effect of M3 mutation on PIN7 trafficking and root gravitropism. (A) Representative confocal fluorescence images of PIN7 (ProPIN7:PIN7:GFP) and M3PIN7 (M3, ProPIN7:M3PIN7:GFP) in root columella cells. Images were taken before (0 min) or after 90°-gravity stimulation for 15 min. Arrows indicate the gravity direction Bar = 20 μm. (B) Fluorescence images of PIN7 and M3PIN7 in root columella regions after 40-min gravity stimulation. Arrow indicates the gravity direction. Red signals were from FM4-64 staining. Bar = 20 μm. (C) The lateral/basal (L/B as indicated in B) ratio of PIN7 or M3PIN7 fluorescence intensity in columella cells at 0 min and after 40 min gravity stimulation. Data represent means ± SE (n = 12 cells from 6 roots for each construct). Asterisks indicate that differences are significant (P < 0.0001) in t-test. The difference of M3 values between 0 and 40 min was not significant (P = 0.631). (D) Kinetics of root gravitropism of WT, homozygous pin7, complemented with PIN7 (PIN7) and M3 (M3PIN7), and homozygous pin7. Data represent means ± SE (n = 29–40 roots). (E) Representative root images of the plants described in (D) after a 10-h gravity stimulus. The arrow indicates the gravity (g) direction.