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In vitro phosphorylation assay of the hydrophilic loop (HL) of PIN1. Autoradiogram (AR) of PIN1-HL phosphorylation assay. Wild-type (WT), M3, and 3m1 PIN1-HL proteins were analyzed for phosphorylation by PID kinase using [γ-32P] ATP as phosphate source. KB, kinase buffer; CBB, Coomassie Brilliant Blue-stained gel. The numbers below AR indicate relative phosphorylation intensities of PIN1 that were normalized by the PIN1 band intensities in CBB.
