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. 2016 Sep 28;6:113. doi: 10.3389/fcimb.2016.00113

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Copyright © 2016 Li and Faucher.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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LasM is expressed in water and is regulated by RpoS. (A) The expression of lasM based on a transcriptomic analysis of the WT JR32 strain exposed to water for 2, 6, and 24 h compared to the control grown to exponential phase in rich medium (adapted from Li et al., 2015 published under Creative Commons Attribution 4.0 International License; https://creativecommons.org/licenses/by/4.0/). (B) The RpoS-dependant regulation of lasM in Lp exposed to water for 6 h was assessed by RT-qPCR. The expression of lasM in the rpoS mutant was compared to that in the WT strain. The expression of lasM in the complemented strain where rpoS is induced with 0.5 mM IPTG (prpoS ON) was compared to that of the non-induced complement (prpoS OFF). One-tailed unpaired Student's t-test was used to assess significant differences between the two ratios (^***p < 0.0005). Data shown are the mean and SD of 3 biological replicates.