Table 1.
Rat transcript cDNA PCR primers and conditions
| Gene | PubMed accession no | Forward primer and start position for first variant | Amplicon length | Annealing temperature (°C) | Melting temperature (°C) |
|---|---|---|---|---|---|
| Reverse primer and start position for first variant | |||||
| PTGS1 (COX1) | NM_017043.3 | F 5′-AAAGAACCCAATGTCCAGCAAG; 2555 bp | 152 bp | 60.8 | 80.9 |
| R 5′-GGCTCCCAACCAAAATCGTAG; 2706 bp | |||||
| PTGS2 (COX2) | NM_017232.3 | F 5′-TTCCAGTATCAGAACCGCATTG; 1069 bp | 151 bp | 60.8 | 80.3 |
| R 5′-CAGCAAGTCCGTGTTCAAGGA; 1219 | |||||
| PGES1 (Microsomal PGE-synthase-1) | NM_021583.2 | F 5′-GCAACGACATGGAGACGATCT; 263 bp | 203 bp | 58 | 84.2 |
| R 5′-CATGGAGAAACAGGCGAACTG; 465 bp | |||||
| Mgll (MAG Lipase) | NM_138502.2 | F 5′-CATGGAGCTGGGGAACACTG; 262 bp | 239 bp | 58 | 81 |
| R 5′-GGAGATGGCACCGCCCATGGAG; 501 bp | |||||
| PTGER2 (EP2) | NM_031088 | F 5′-CTTGCTCTTCTGTTCTCTGCCG; 537 bp | 205 bp | 60.8 | 85 |
| R 5′-GCTTCTTTTGCTCCGAAGCTG; 741 bp | |||||
| PTGER3 (EP3) | NM_012704 | F 5′-TTGTGTCGCGCAGCTATAGAC; 231 bp | 209 bp | 60 | 87 |
| R 5′-TCCGAACACTGTCATGGTCAG; 429 bp | |||||
| PTGER4 (EP4) | NM_032076 | F 5′-TGCTCAGAGAGTCGGAGGACAAT; 1276 bp | 215 bp | 60 | 84.5 |
| R 5′-GAAATTCGCAAAGTTCTCAGCG; 1490 bp | |||||
| CYP19A1 (Aromatase) | NM_017085.2 | F 5′-AACGTGAATCAGTGTATATTGGAAATG; 1495 bp | 67 bp | 56 | 77.5 |
| R 5′-CGTACAGAGTGACGGACATGG; 1561 bp | |||||
| ESR1 (ERα) | NM_012689.1 | F 5′-GAAAGGCGGGATACGAAAAGA; 977 bp | 59 bp | 60 | 76.8 |
| R 5′- TCTGACGCTTGTGCTTCAACA; 1035 bp | |||||
| ESR2 (ERβ) | NM_012754.1 | F 5′-TCTGTCCAGCCACGAATCAG; 1035 bp | 59 bp | 60 | 79.1 |
| R 5′-GGCCTGGCAGCTTTTACG; 1093 bp | |||||
| GAPDH | NM_017008.4 | F 5′-TGGTGAAGGTCGGTGTGAACGG; 77 bp | 70 bp | 56–60.8 | 80.9 |
| R 5′-TCACAAGAGAAGGCAGCCCTGGT; 146 bp |