Figure 3.

Systemic administration of alcohol, moderate alcohol intake, or sucrose consumption do not alter BDNF receptors expression or localization in the DLS. A, B, Rats received a systemic administration of alcohol (Alc; 1.5 g/kg, i.p.; black) or saline (Sal; white), and the DLS was dissected 30 min later. C, D, Rats received continuous access to 10% alcohol (CA10%; black) or water only (white) for 21 d, and the DLS was dissected immediately after the last drinking session. E, F, Rats received 7 weeks of IA of 1% sucrose (Suc) or water only, and the DLS was dissected immediately after the last 30 min drinking session. TrkB and p75NTR levels in total homogenate (A, C, E) or in the synaptosomal fraction (B, D, F) were measured by Western blot analysis. Histograms show mean ratio of TrkB or p75NTR to actin ± SEM, and values are expressed as percentages of water controls. n = 8 per treatment (A, B); n = 8 (C, D) and 4 per drinking regimen (E, F).