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. 2016 Sep 28;36(39):10151–10162. doi: 10.1523/JNEUROSCI.0845-16.2016

Figure 9.

Figure 9.

Constitutively active EphB2 kinase restores BLA neuron maturation and innate fear responses in ephrin-B3-null mice. A, EphB2−/− and EphB2lacZ/lacZ but not EphB2F620D/F620D mice spent significantly more time in the open arms than wild types at P16. n = 14–18 mice for per group. B, C, Behavioral response of innate fear in the EPM and c-Fos activation in BLA and CA1 neurons from Efnb3−/−; EphB2+/+ and Efnb3−/−; EphB2F62D/F620D mutants were quantified. n = 5–7 mice for per group. D, Spine morphogenesis in BLA neurons from Efnb3−/−; EphB2+/+ and Efnb3−/−; EphB2F62D/F620D mutants at P16. Quantification of the densities for total and mushroom spines (arrowheads) in Efnb3−/−; EphB2+/+ and Efnb3−/−; EphB2F62D/F620D mutants was performed at P16. n = 19–25 neurons for per group. E, Summary model of EphB2 signal-mediated spinogenesis to generate innate fear responses. The presynaptic ephrin-B3 guides hippocampal axons to wire EphB2 receptor-expressing neurons in amygdala during the critical period. EphB2 receptor kinase activation consolidates the transsynaptic connection that is required for mushroom-type spinogenesis and threat-stimulated defensive responses in BLA neurons. Scale bars: C, 100 μm; D, 5 μm. Data are presented as mean ± SEM. *p < 0.05.