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. 2016 Sep 28;36(39):10214–10227. doi: 10.1523/JNEUROSCI.0226-16.2016

Figure 5.

Figure 5.

Monaural conductive hearing loss increases GluA3 AMPAR subunits at the synapse of the auditory nerve on bushy cells. A, Examples of high-magnification electron micrographs of auditory nerve synapses on bushy cells that were immunogold labeled (10-nm-diameter gold particles) for GluA2/3, GluA2, and GluA4 from sham and earplugged animals at P40. AN, Auditory nerve; BC, bushy cell. Scale bar, 50 nm. B, Distribution of GluA2/3, GluA2, and GluA4 gold particles per PSD of the synapse of the auditory nerve on bushy cells from sham and earplugged (EP) animals. GluA2/3 labeled/total #PSD: sham, 125/155; EP, 125/144; GluA2 labeled/total #PSD: sham, 48/112; EP, 46/106; GluA4 labeled/total #PSD: sham, 44/112; EP, 32/112. C, Plots of the mean number and density of gold particles showing that GluA2/3 gold labeling was increased in earplugged (EP) animals (number: sham, 3.21 ± 0.23; EP, 3.80 ± 0.21; p = 0.0132, Mann–Whitney U test; density: sham, 12.52 ± 0.66; EP, 10.51 ± 0.68; p = 0.0122, Mann–Whitney U test). Gold labeling for GluA2 and GluA4 was similar between sham and EP animals (GluA2 number: sham, 0.61 ± 0.08; EP, 0.68 ± 0.11; p = 0.8389, Mann–Whitney U test; density: sham, 2.05 ± 0.26; EP, 2.29 ± 0.35; p = 0.9374, Mann–Whitney U test; GluA4 number: sham, 0.64 ± 0.13; EP, 0.42 ± 0.07; p = 0.1690, Mann–Whitney U test; density: sham, 2.1 ± 0.40; EP, 1.47 ± 0.26; p = 0.1603, Mann–Whitney U test). Error bars are ±SEM. #GP, Number of gold particles per PSD.