Diagram summary of the data obtained in this study. After 10 d of earplugging, there was a decrease in vGluT1 labeling at auditory nerve (AN) endings and in the size of SVs. Postsynaptically, the PSDs of the AN on bushy cell (BC) somata were thicker and contained increased GluA3 AMPAR subunits. Ten days after earplug removal, there was an increase in the density of SVs in the AN; SVs were also larger in size. On BCs, the PSD apposed to the AN were larger and thicker, and they contained fewer GluA2 but more GluA3 subunits.