Figure 4.

Maximum intensity projections of confocal z-stacks taken from retinal whole-mounts showing the co-localization of THY1 (A) and Class III beta-tubulin (B) in the retinal ganglion cell layer from a C57BL/6 mouse. Most cells were double-stained for both RGC markers (C), but some cells only expressed Class III beta-tubulin (arrowhead) or THY1 (arrow). Furthermore, the staining intensity was different across cells, and some cells had large somata and were more intensely stained than others (large arrowhead in “merge”). Staining with the secondary antibodies only did not result in unspecific fluorescence (data not shown). Scale bar in C = 100 μm.