Skip to main content
. 2016 Aug 18;6(11):1975–1987. doi: 10.7150/thno.16547

Figure 3.

Figure 3

Calcium content of aorta 30 days after injury and before any treatment compared to EL-NP-Blank group, EL-NP-BB94 group, EL-NP-PGG group and EL-NP-EDTA+EL-NP-PGG groups (#P < 0.05, Tukey's test,) (n=6). Alizarin red staining (calcium) with a Light Green SF Yellowish counterstain for the EL-NP-Blank group (B1), EL-NP-BB94 group (B2), EL-NP-PGG group (B3), and EL-NP-EDTA+EL-NP-PGG group (B4) shows high calcification (B1, B2); mild calcification (B3); minimal calcification (B4). OPN IHC revealed higher OPN expression in the EL-NP-Blank and EL-NP-BB94 groups (C1, C2); less OPN expression in the EL-NP-PGG group and EL-NP-EDTA+EL-NP-PGG groups (C3, C4). For histological confirmation of PGG binding to elastic lamina, all groups' sections were stained with a phenol-specific stain (black indicates PGG) and counterstained with Light Green SF Yellowish, showing no staining for EL-NP-Blank (D1) and EL-NP-BB94 (D2); mild phenol staining close to the elastic lamina in the EL-NP-PGG group (D3); very strong staining in the EL-NP-EDTA+EL-NP-PGG group (D4). * indicates lumen. Scale bar is 100 µm.